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A. TpPK without and with 0.2 mM Mn2+ are represented by solid and dashed-dotted lines, respectively. B. TpPK: solid line, RMPK: dashed line, C. TpPK: solid line, the B domain: dotted line. The enzyme concentration was 1.0 mg/ml (19.47 μM monomer) for TpPK, the B domain concentration was 0.18 mg/ml (19.55 μM of domain). The scan rate was 1.5°C/min.
