Table 4. Detectability and repeatability assessment in artificially contaminated plant extracts of the duplex-PCR 93F/93R & 5F/5R for Ralstonia solanacearum Moko disease-causing strains and IIB-4NPB strains.
| Strain | Phylotype-Sequevar | Concentration (CFU/mL) 1 | |||||
|---|---|---|---|---|---|---|---|
| 106 | 105 | 104 | 103 | 102 | 101 | ||
| GMI8044 | IIA-6 | +++/+++ | +++/+++ | -++/-++ | —-/—+ | —-/—- | —-/—- |
| 9–1 | IIA-6 | +++/+++ | +++/+++ | -++/—+ | +—/—- | —-/—- | —-/—- |
| A3909 | IIA-6 | +++/+++ | +++/+++ | +—/+— | —-/—- | —-/—- | —-/—- |
| IBSBF1900 | IIA-24 | +++/+++ | +++/+++ | -++/-++ | -+-/—- | —-/—- | —-/—- |
| B50 | IIA-24 | +++/+++ | +++/+++ | +-+/+— | +—/—- | —-/—- | —-/—- |
| B26 | IIA-24 | +++/+++ | +++/+++ | ++-/++- | —-/—- | —-/—- | —-/—- |
| CIP417 | IIB-3 | +++/+++ | +++/+++ | —+/-++ | —+/—+ | —-/—- | —-/—- |
| CFBP1416 | IIB-3 | +++/+++ | +++/+++ | -+-/-+- | —-/—- | —-/—- | —-/—- |
| MOLK2 | IIB-3 | +++/+++ | +++/+++ | +—/+— | +—/—- | —-/—- | —-/—- |
| UW160 | IIB-4 | +++/+++ | +++/+++ | —-/—- | —-/—- | —-/—- | —-/—- |
| UW163 | IIB-4 | +++/+++ | +++/+++ | +—/+— | —-/—- | —-/—- | —-/—- |
| UW179 | IIB-4 | +++/+++ | +++/+++ | -++/-++ | —-/—- | —-/—- | —-/—- |
| ANT80 | IIB-4NPB | +++/+++ | +++/+++ | -+-/-+- | -+-/-+- | —-/—- | —-/—- |
| CFBP6780 | IIB-4NPB | +++/+++ | +++/+++ | —+/—+ | —-/—- | —-/—- | —-/—- |
| CFBP6783 | IIB-4NPB | +++/+++ | +++/+++ | +—/+— | -+-/-+- | —-/—- | —-/—- |
| CFBP6797 | IIB-4NPB | +++/+++ | +++/+++ | —-/—- | —-/—- | —-/—- | —-/—- |
| CFBP7014 | IIB-4NPB | +++/+++ | +++/+++ | ++-/++- | ++-/++- | —-/—- | —-/—- |
| IBSBF1454 | IIB-4NPB | +++/+++ | +++/+++ | —-/—- | —-/—- | —-/—- | —-/—- |
| IBSBF1503 | IIB-4NPB | +++/+++ | +++/+++ | +—/+— | +—/+— | —-/—- | —-/—- |
| LNPV24.25 | IIB-4NPB | +++/+++ | +++/+++ | —+/—+ | —+/—+ | —-/—- | —-/—- |
| LNPV30.75 | IIB-4NPB | +++/+++ | +++/+++ | -++/-++ | —-/—- | —-/—- | —-/—- |
| PV1 | IIB-4NPB | +++/+++ | +++/+++ | —-/—- | —-/—- | —-/—- | —-/—- |
| Detectability 2 | 100% | 100% | 41% | 18% | 0% | 0% | |
| Repeatability 2 | 100% | 100% | 95% | 92% | 100% | 100% | |
1 ‘+’: positive result; ‘-’: negative result; two PCR reactions per sample were repeated three times (separated by a slash)
2 calculated according to the ratio of agreements relatively to the total repetitions performed within the experiment (agreements and disagreements).