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. 2015 Mar 26;10(3):e0120895. doi: 10.1371/journal.pone.0120895

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© 2015 Pirilä et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — Vybrant CM-Dil labeled HSC-3 (red) and Vybrant DiO labeled Mfs (green) were co-cultured for up to 11 days in normal growth medium and photographed with an Evos FL Cell Imaging System microscope. Cell density was analyzed optically using Leica QWin3 Software. Co-culture of labeled HSC-3 and M1 Mfs (A-C), HSC-3 and M2 Mfs (D-F) and HSC-3 and R848 Mfs (G-I) at days 2, 5 and 7. White arrows shows merged/fused cells. (J) Relative HSC-3 cell density in co-cultures. Scale bars 400 μm and n = 2. Representative confocal microscope pictures of co-cultured HSC-3 and R848 Mfs are shown in K-M. Fusion of membranes was observed between the two cell types (white arrows). An R848 macrophage appears to deliver parts to surrounding HSC-3 cells (L), larger picture in M. Scale bars 50 μm in K,L and 25 μm in M.