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. 2015 Mar 26;10(3):e0120895. doi: 10.1371/journal.pone.0120895

Fig 2. Efferocytosis in HSC-3 and Mf co-cultures.

Fig 2

Vybrant CM-Dil labeled HSC-3 (red) and Vybrant DiO-labeled M2 Mfs (green) were co-cultured, treated with 3 mM efferocytosis-inhibitor amiloride (AMI) or 10 μM NF-κB inhibitor BAY 11-7082. Co-cultures of HSC-3 cells and M2 Mfs were photographed with an EVOS FL Cell Imaging System microscope. Pictures presented are from day 2 (n = 4). Fusion of membranes was observed between HSC-3 cells and M2 Mfs in DMSO (vehicle)- and BAY-treated cultures (white arrows in Overlay-section). Scale bars 200 μm.