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. 2015 Mar 26;10(3):e0120895. doi: 10.1371/journal.pone.0120895

Fig 9. Summary of NF-κB translocation/activation in HSC-3 cell and Mfs co-cultures.

Fig 9

In monocultures, a typical activation of the NF-κB dimer (p65/p50 translocation to nucleus) is seen in HSC-3 cells and M1 Mfs while M2 Mfs present with a p50/p50 homodimer pattern also present in R848 Mfs. In co-cultures, HSC-3 cells induced a shift in M1 Mfs NF-κB activation towards a p50/p50 profile. In HSC-3 and M2 Mfs co-cultures on the other hand, NF-κB activation in the HSC-3 cells was shifted if the HSC-3 cells were in a close proximity to the M2 Mfs. The NF-κB activation profile of R848 Mfs in co-cultures was more cryptic but translocation of p50/p65 could be observed. The p65/p50 heterodimer activates NF-κB inducible genes while the p50/p50 homodimer is associated with repressed gene transcription or induction of tumor-progressive genes.