Fig 3. SU86.86 cells exhibit phenotypic and genotypic changes following selection by IH.

SU86.86 cells were treated with IH for 50 cycles, had clonal populations raised, and grown in normoxia for 2 months. A significant decrease in expression of E-cadherin (A) and p53 (B) was detected by quantitative Real-Time PCR. IH-selected SU86.86 clones also exhibited an increase in resistance to etoposide (C) and hypoxia (D).Results of selection of RKO cells following IH-selection (E-H). We do not detect any changes in p53 or E-cadherin expression within clones of RKO IH-selected (E,F). Furthermore, these cells did not exhibit resistance to etoposide (G), nor hypoxia (H).