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. 2015 Mar 26;10(3):e0120139. doi: 10.1371/journal.pone.0120139

Fig 2. Chromosome walking of the gadA locus of Lactobacillus brevis NCL912 (a), human aldolase A gene (b), malQ of Pichia pastoris GS115 (c), and hyg of rice (d).

Fig 2

I: walking into 5’ regions of the genes (locus); II: walking into 3’ regions of the genes (locus). Each walking experiment contained four sets of PCRs that respectively utilized the four POP primer sets, POP1, POP2, POP3, and POP4, paired with a specific primer set. For each set of PCRs, only the results of secondary PCR (left lane) and tertiary (right lane) PCR are presented. White arrows indicate target bands. M1: DL2000 DNA marker. M2: λ-Hind III digest DNA Marker. M3: DL5000 DNA marker.