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. 2015 Mar 26;10(3):e0122297. doi: 10.1371/journal.pone.0122297

Fig 5. VLCAD from SIRT3 and SIRT5 knockout mice shows reduced affinity for cardiolipin.

Fig 5

A) The fat blot method was used to evaluate endogenous VLCAD binding to cardiolipin in fasted (20 hr) mouse liver lysates. B) Densitometry was used to quantify binding from panel A. C) Lysate from VLCAD-/- liver was tested as a negative control and shows no detectable signal. D) Western blot was used to confirm that total VLCAD expression is not significantly different between wild-type, SIRT3 KO, and SIRT5 KO mice.