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. 2015 Mar 26;10(3):e0121751. doi: 10.1371/journal.pone.0121751

Fig 3. ELISA reactivity of MCPyV monoclonal antibodies against MCPyV insertional mutants BC, DE, EF and HI.

Fig 3

In order to characterize the epitopes of the MCPyV VP1, the reactivity of mAbs was analyzed using the MCPyV wt VP1 VLPs and the four mutants with insertion within the BC, DE, EF and HI VP1 loops. ELISAs were performed using hybridoma culture supernatants diluted 1:3. The results are presented as relative binding defined as the reactivity of mAb to mutant VLPs divided by the reactivity of the same mAb observed with wild-type VLPs. The data presented are the means of three determinations (+/- SEM).