Table 1. Sequence of primers used for generation of MCPyV VP1 mutants ^a .

Name	5’→3’	Restriction site
5’ full VP1	GGATCCCCTGAATTACAAGTAATTGAAGATGGCACC	BamHI
3’ full VP1	AAGCTTCTGAATAGGAATGCATGAAATAATTCTCAT	HindIII
5’ BC loop	TGGAGCCATCCGCAATTTGAAAAGTCTCCAGATCAGCCCATCAAGG
3’ BC loop	CTTTTCAAATTGCGGATGGCTCCATGATCCCTTTGGCTGCAGGTCATAAG
5’ DE loop	TGGAGCCATCCGCAATTTGAAAAGGATTACGGTGCTGGTATTCC
3’ DE loop	CTTTTCAAATTGCGGATGGCTCCAATGAACTCTTTTCATGTCCC
5’ EF loop	TGGAGCCATCCGCAATTTGAAAAGACAAATGGTGGGCCTATTACAATTG
3’ EF loop	CTTTTCAAATTGCGGATGGCTCCAAGTTTTTGGATACTCAGTCTGGTAATC
5’ HI loop	TGGAGCCATCCGCAATTTGAAAAGAGTGGAAAAATGGCTCTTCATGGG
3’ HI loop	CTTTTCAAATTGCGGATGGCTCCAGGTTTTAAACAGAAACCCCACTATG

^a Restriction site sequences are italicized