Table 3.
Functional classification of extracted coding sequences (CDS) from the putative secreted class from the sialotranscriptome of adult Rhipicephalus pulchellus ticks.
| Class | CDS | Associated Reads | % Total reads |
|---|---|---|---|
| Enzymes | |||
| Metalloproteases | |||
| Typical tick ZnMc_salivary_gland_MPs CDD motif | 98 | 180,020 | 1.1195 |
| Large ADAM's proteases - probably housekeeping | 5 | 4,059 | 0.0252 |
| Other Zn metalloproteases, including collagenases | 7 | 6,128 | 0.0381 |
| M13 family peptidase | 14 | 25,327 | 0.1575 |
| Dipeptidyl peptidase | 2 | 13,064 | 0.0812 |
| Serine protease | 17 | 12,921 | 0.0804 |
| Male specific salivary serine protease | 2 | 32,135 | 0.1998 |
| Suchi serine protease | 2 | 3,421 | 0.0213 |
| Zinc carboxypeptidase | 2 | 9,147 | 0.0569 |
| Serine carboxypeptidase - may be lysosomal | 19 | 43,212 | 0.2687 |
| Legumain/asparaginyl peptidase | 2 | 6,916 | 0.0430 |
| Chitinase | 2 | 2,106 | 0.0131 |
| 5'-nucleotidase/Apyrase | 20 | 28,812 | 0.1792 |
| Ribonuclease | 4 | 4,892 | 0.0304 |
| Ectonucleotide pyrophosphatase/phosphodiesterase family | 3 | 5,489 | 0.0341 |
| Lipase | 4 | 2,591 | 0.0161 |
| Inositol phosphatase | 4 | 3,734 | 0.0232 |
| Antigen 5 family | 20 | 65,252 | 0.4058 |
| Proteinase inhibitor domains | |||
| Serpins | 23 | 10,468 | 0.0651 |
| Monolaris | 81 | 125,953 | 0.7833 |
| Bilaris | 65 | 94,581 | 0.5882 |
| Trilaris | 33 | 37,042 | 0.2304 |
| Tetralaris | 6 | 32,425 | 0.2016 |
| Pentalaris | 4 | 44,321 | 0.2756 |
| Hexalaris | 1 | 368 | 0.0023 |
| Heptalaris | 2 | 4,770 | 0.0297 |
| Similar to Kunitz domain | 10 | 10,995 | 0.0684 |
| Cystatins | 20 | 73,488 | 0.4570 |
| Thyropins with two domains | 4 | 2,786 | 0.0173 |
| IGF/KAZAL/IG domain family | 2 | 2,019 | 0.0126 |
| Chymotrypsin-elastase inhibitor ixodidin - TIL domain | 33 | 636,225 | 3.9564 |
| MonoTil | 13 | 26,055 | 0.1620 |
| BiTil | 35 | 96,191 | 0.5982 |
| TriTil | 12 | 50,388 | 0.3133 |
| PolyTil | 2 | 12,311 | 0.0766 |
| Til-Like | 1 | 31 | 0.0002 |
| Immunity associated products | |||
| Lysozyme | 4 | 2,397 | 0.0149 |
| Defensins | 22 | 5,555 | 0.0345 |
| TEP proteins | 23 | 50,188 | 0.3121 |
| ML domaini | 9 | 1,533 | 0.0095 |
| Galectin | 6 | 7,046 | 0.0438 |
| Peptidoglycan recognition protein | 6 | 7,098 | 0.0441 |
| Ixoderin/Ficolin | 6 | 17,076 | 0.1062 |
| Tick specific protein families, at least one family member has known function | |||
| Antimicrobial peptides | |||
| Microplusin | 6 | 14,525 | 0.0903 |
| 5.3 kDa antimicrobial family | 4 | 317 | 0.0020 |
| Protease inhibitors | |||
| Carboxypeptidase inhibitor | 13 | 17,373 | 0.1080 |
| Basic Tail | 12 | 18,408 | 0.1145 |
| 18.3 kDa family | 14 | 12,823 | 0.0797 |
| Glycine rich superfamily | 151 | 7,640,865 | 47.5157 |
| Mucins | 118 | 1,840,933 | 11.4481 |
| Lipocalin family | |||
| Group I | 209 | 309,678 | 1.9258 |
| Group II | 20 | 34,862 | 0.2168 |
| Group III | 13 | 45,186 | 0.2810 |
| Group IV | 10 | 24,295 | 0.1511 |
| Group V | 16 | 25,277 | 0.1572 |
| Group VI | 13 | 176,753 | 1.0992 |
| Group VII | 17 | 17,945 | 0.1116 |
| Group VIII - Deorphanized Dermacentor lipocalin family | 19 | 20,816 | 0.1294 |
| New lipocalin family Rhipicephalus family XII | 5 | 7,271 | 0.0452 |
| Other putative lipocalins | 64 | 912,901 | 5.6770 |
| Ixodegrins | 21 | 45,029 | 0.2800 |
| DA-P36 family | 31 | 36,142 | 0.2248 |
| Evasin | 22 | 19,746 | 0.1228 |
| Immunoglobulin G binding protein | 12 | 1,382,686 | 8.5984 |
| Function Unknown | |||
| Found in prostriates and metastriates | |||
| Fibronectin domain containing protein family | 2 | 157 | 0.0010 |
| Hematopoietic-stem cell progenitor | 2 | 1,551 | 0.0096 |
| 8.9 kDa family | 60 | 114,278 | 0.7107 |
| 23 kDa family | 7 | 3,072 | 0.0191 |
| 24 kDa family | 9 | 3,193 | 0.0199 |
| One of each protein family - now Ixodidae | 14 | 3,505 | 0.0218 |
| Previously thought as metastriate specific | |||
| Ig domain containing secreted peptide | 4 | 1,814 | 0.0113 |
| Deorphanized Ixodidae Rhip 45-937 | 4 | 1,901 | 0.0118 |
| Insulin growth factor binding protein - also found in Ixodes | 6 | 2,065 | 0.0128 |
| Amblyomma maculatum family 40-33 | 9 | 2,599 | 0.0162 |
| Deorphanized metastriate-Rhipicephalus family XIV | 4 | 912 | 0.0057 |
| Deorphanized metastriate family 40-80 | 1 | 626 | 0.0039 |
| Somatomedin domain protein family | 4 | 599 | 0.0037 |
| Metastriate specific | |||
| 28 kDa Metastriate family | 18 | 17,340 | 0.1078 |
| 16 kDa family | 2 | 8,950 | 0.0557 |
| 8 kDa Amblyomma family | 5 | 2,135 | 0.0133 |
| 10 kDa acidic metastriate | 1 | 6,626 | 0.0412 |
| Ixostatin-like | 10 | 12,781 | 0.0795 |
| Deorphanized Dermacentor 9 kDa expansion | 5 | 1,102 | 0.0069 |
| Rhip 45-304 - deorphanized on A. maculatum sialome | 4 | 13,864 | 0.0862 |
| Rhipicephalus specific families | |||
| Rhip 45-141 | 17 | 2,401 | 0.0149 |
| Rhip 45-151 | 15 | 3,199 | 0.0199 |
| Rhip 45-236 | 2 | 22,601 | 0.1405 |
| Short protein families possibly secreted | |||
| Rhip 50-70 | 6 | 1,431 | 0.0089 |
| Rhip 50-10 | 107 | 16,133 | 0.1003 |
| Rhip 50-45 | 26 | 21,670 | 0.1348 |
| Rhip 50-18 | 79 | 10,396 | 0.0646 |
| Rhip 45-25 | 64 | 21,119 | 0.1313 |
| Rhip 45-85 | 16 | 1,445 | 0.0090 |
| Rhip 40-92 | 23 | 2,214 | 0.0138 |
| Rhip 45-2 | 623 | 179,776 | 1.1180 |
| Rhip 45-10 | 43 | 15,020 | 0.0934 |
| Rhip 45-11 | 162 | 36,474 | 0.2268 |
| Rhip 45-16 | 105 | 21,708 | 0.1350 |
| Rhip 45-22 | 56 | 13,453 | 0.0837 |
| Rhip 45-27 | 62 | 13,982 | 0.0869 |
| Rhip 45-28 | 53 | 41,876 | 0.2604 |
| Rhip 45-29 | 56 | 11,234 | 0.0699 |
| Rhip 45-37 | 43 | 2,067 | 0.0129 |
| Rhip 45-48 | 37 | 7,106 | 0.0442 |
| Rhip 45-49 | 35 | 4,196 | 0.0261 |
| Rhip 45-56 | 33 | 8,522 | 0.0530 |
| Rhip 45-61 | 30 | 52,808 | 0.3284 |
| Rhip 45-66 | 28 | 2,827 | 0.0176 |
| Rhip 45-69 | 27 | 11,930 | 0.0742 |
| Rhip 45-72 | 26 | 4,828 | 0.0300 |
| Rhip 45-73 | 26 | 2,433 | 0.0151 |
| Rhip 45-77 | 25 | 11,873 | 0.0738 |
| Rhip 45-82 | 24 | 2,064 | 0.0128 |
| Rhip 45-95 | 22 | 5,455 | 0.0339 |
| Rhip 45-130 | 18 | 2,533 | 0.0158 |
| Rhip 45-132 | 18 | 771 | 0.0048 |
| Rhip 45-133 | 18 | 1,524 | 0.0095 |
| Rhip 45-143 | 17 | 3,894 | 0.0242 |
| Rhip 45-144 | 17 | 3,781 | 0.0235 |
| Remaining peptides of large small family 40-1 | 3,340 | 619,033 | 3.8495 |
| Other secreted proteins - includes fragments | 208 | 272,152 | 1.6924 |
| Conserved secreted protein | 16 | 21,361 | 0.1328 |
| Total | 7,134 | 16,080,727 | 100 |