Table 7.
Functional classification of secretory R. pulchellus salivary gland proteins identified in the proteome
| All proteins* | Found in both sexes | Found in males only | Found in females only | |||||
|---|---|---|---|---|---|---|---|---|
| Class | Number | % of total | Number | % of total | Number | % of total | Number | % of total |
| Enzymes | ||||||||
| Metalloproteases | 6 | 2.71 | 1 | 3.23 | 1 | 1.30 | 4 | 3.54 |
| M13 family peptidase | 2 | 0.90 | 2 | 6.45 | 0 | 0.00 | 0 | 0.00 |
| Dipeptidyl peptidase | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Serine protease | 3 | 1.36 | 0 | 0.00 | 2 | 2.60 | 1 | 0.88 |
| Male specific salivary serine protease | 2 | 0.90 | 0 | 0.00 | 2 | 2.60 | 0 | 0.00 |
| Zinc carboxypeptidase | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Serine carboxypeptidase - may be lysosomal | 10 | 4.52 | 0 | 0.00 | 9 | 11.69 | 1 | 0.88 |
| Legumain/asparaginyl peptidase | 2 | 0.90 | 0 | 0.00 | 1 | 1.30 | 1 | 0.88 |
| 5'-nucleotidase/Apyrase | 8 | 3.62 | 2 | 6.45 | 2 | 2.60 | 4 | 3.54 |
| Ribonuclease | 2 | 0.90 | 0 | 0.00 | 0 | 0.00 | 2 | 1.77 |
| Inositol phosphatase | 2 | 0.90 | 0 | 0.00 | 0 | 0.00 | 2 | 1.77 |
| Antigen 5 family | 1 | 0.45 | 0 | 0.00 | 1 | 1.30 | 0 | 0.00 |
| Proteinase inhibitor domains | ||||||||
| Serpins | 7 | 3.17 | 1 | 3.23 | 5 | 6.49 | 1 | 0.88 |
| Monolaris | 2 | 0.90 | 0 | 0.00 | 1 | 1.30 | 1 | 0.88 |
| Bilaris | 5 | 2.26 | 0 | 0.00 | 0 | 0.00 | 5 | 4.42 |
| Tetralaris | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Similar to Kunitz domain | 1 | 0.45 | 0 | 0.00 | 1 | 1.30 | 0 | 0.00 |
| Cystatins | 2 | 0.90 | 0 | 0.00 | 2 | 2.60 | 0 | 0.00 |
| Chymotrypsin-elastase inhibitor ixodidin - TIL domain | 1 | 0.45 | 0 | 0.00 | 1 | 1.30 | 0 | 0.00 |
| BiTil | 11 | 4.98 | 2 | 6.45 | 5 | 6.49 | 4 | 3.54 |
| TriTil | 8 | 3.62 | 0 | 0.00 | 8 | 10.39 | 0 | 0.00 |
| Immunity associated products | ||||||||
| Defensins | 1 | 0.45 | 0 | 0.00 | 1 | 1.30 | 0 | 0.00 |
| TEP proteins | 4 | 1.81 | 0 | 0.00 | 0 | 0.00 | 4 | 3.54 |
| Galectin | 3 | 1.36 | 0 | 0.00 | 0 | 0.00 | 3 | 2.65 |
| Tick specific protein families, at least one family member has known function | ||||||||
| Antimicrobial peptides | ||||||||
| Microplusin | 3 | 1.36 | 0 | 0.00 | 1 | 1.30 | 2 | 1.77 |
| Protease inhibitors | ||||||||
| Carboxypeptidase inhibitor | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Basic Tail | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Glycine rich superfamily | 14 | 6.33 | 3 | 9.68 | 2 | 2.60 | 9 | 7.96 |
| Mucins | 6 | 2.71 | 0 | 0.00 | 4 | 5.19 | 2 | 1.77 |
| Lipocalin family | ||||||||
| Group I | 34 | 15. 38 | 7 | 22. 58 | 13 | 16. 88 | 14 | 12. 39 |
| Group II | 3 | 1.36 | 1 | 3.23 | 0 | 0.00 | 2 | 1.77 |
| Group III | 4 | 1.81 | 0 | 0.00 | 0 | 0.00 | 4 | 3.54 |
| Group IV | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Group V | 3 | 1.36 | 0 | 0.00 | 0 | 0.00 | 3 | 2.65 |
| Group VI | 2 | 0.90 | 1 | 3.23 | 0 | 0.00 | 1 | 0.88 |
| Group VIII - Deorphanized Dermacentor lipocalin family | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| New lipocalin family | ||||||||
| Rhipicephalus family XII | 2 | 0.90 | 0 | 0.00 | 2 | 2.60 | 0 | 0.00 |
| Other putative lipocalins | 13 | 5.88 | 4 | 12.90 | 4 | 5.19 | 5 | 4.42 |
| Ixodegrins | 3 | 1.36 | 0 | 0.00 | 0 | 0.00 | 3 | 2.65 |
| DA-P36 family | 7 | 3.17 | 3 | 9.68 | 1 | 1.30 | 3 | 2.65 |
| Immunoglobulin G binding protein | 1 | 0.45 | 0 | 0.00 | 1 | 1.30 | 0 | 0.00 |
| Function Unknown | ||||||||
| Found in prostriates and metastriates | ||||||||
| Hematopoietic-stem cell progenitor | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| 8.9 kDa family | 7 | 3.17 | 0 | 0.00 | 4 | 5.19 | 3 | 2.65 |
| 24 kDa family | 1 | 0.45 | 1 | 3.23 | 0 | 0.00 | 0 | 0.00 |
| One of each protein family - now Ixodidae | 4 | 1.81 | 0 | 0.00 | 0 | 0.00 | 4 | 3.54 |
| Metastriate specific | ||||||||
| 28 kDa Metastriate family | 4 | 1.81 | 1 | 3.23 | 1 | 1.30 | 2 | 1.77 |
| 10 kDa acidic metastriate | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Ixostatin-like | 2 | 0.90 | 2 | 6.45 | 0 | 0.00 | 0 | 0.00 |
| Rhip 45-304 - deorphanized on A. maculatum sialome | 1 | 0.45 | 0 | 0.00 | 0 | 0.00 | 1 | 0.88 |
| Rhipicephalus specific families | ||||||||
| Rhip 45-236 | 2 | 0.90 | 0 | 0.00 | 2 | 2.60 | 0 | 0.00 |
| Short protein families possibly secreted | 2 | 0.90 | 0 | 0.00 | 0 | 0.00 | 2 | 1.77 |
| Other secreted proteins - includes fragments | 9 | 4.07 | 0 | 0.00 | 0 | 0.00 | 9 | 7.96 |
| Conserved secreted protein | 2 | 0.90 | 0 | 0.00 | 0 | 0.00 | 2 | 1.77 |
| Total | 221 | 100 | 31 | 100 | 77 | 100 | 113 | 100 |