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. 2015 Mar 26;10(3):e0120956. doi: 10.1371/journal.pone.0120956

Fig 7. Mstn knockdown suppresses the sheep myoblast differentiation.

Fig 7

Sheep myoblasts harboring Myostatin shRNAs and the negative control were cultured 3 days post-infection in DM. (A, B): phase contract photos; (C, D): nuclei stained by DAPI; (E, F): those cells harboring Myostatin shRNAs express eGFP; (G): counts of myotube and DAPI stained nucleus in a given area of view. (H): the fusion index of sheep myoblasts. Results are presented as the mean + SD of five replicates at least. The position of the samples on the plate was randomly assigned. ** P < 0.01, *** P < 0.001.