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. 2015 Mar 26;10(3):e0121313. doi: 10.1371/journal.pone.0121313

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© 2015 Tyagi et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Logarithmic S. aureus ATCC 29213 was labeled with calcein and analyzed for membrane permeabilization after incubation with different concentrations of curcumin I. A total of 10,000 cells were acquired for each flow cytometry analysis. (a) Histograms showing calcein leakage, on treatment with different concentration of curcumin I (0 μM (control), 25 μM, 50 μM & 100 μM), (b) percentage of calcein leakage from S. aureus cells on treatment with different concentration of curcumin I for 2h as quantified from different days of flow cytometry data. These data represents mean (±SD) of three independent experiments (*p ≤ 0.001).