Fig 1. Responsivity of hygromycin-resistant AIZ-AR clones to 100 nM DHT after 24 hours.

Cells were treated for 24 h with 5α-dihydrotestosterone (DHT; 100 nM) and vehicle (DMSO; 0.1% v/v). Luciferase activity was measured in cell lysates. Data are mean from triplicate measurements and are expressed as relative luciferase units (RLU). Fold induction over DMSO-treated cells was calculated and it is indicated above each clone in the bar graph. Similar data were obtained from three consecutive passages.