Table 3. Characteristics of AIZ-AR cells in comparison with published data.
EC50—half maximal effective concentration; IC50—half maximal inhibitory concentration; n.c.—not calculated; n.d.—not determined.
| Compound | Log EC50 | Log IC50 (DHT 100nM) | Log IC50 (Testosterone 10 nM) | Literature Log EC50 | ||||
|---|---|---|---|---|---|---|---|---|
| Clone 8 | Clone 14 | Clone 8 | Clone 14 | Clone 8 | Clone 14 | Sedlak et al. | Wilkinson et al. | |
| 5α-dihydrotestosterone | -9.16 ± 0.09 | -9.19 ± 0.10 | - | - | > -5.00 | > -5.00 | -11.38 ± 0.21 | -9.52 |
| Testosterone | -8.92 ± 0.33 | -9.10 ± 0.09 | > -5.00 | > -5.00 | - | - | -10.00 ± 0.26 | -11.37 |
| Danazol | -8.29 ± 0.34 | -8.13 ± 0.12 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | -9.38 ± 0.17 | -9.47 |
| Cyproterone acetate | -6.69 ± 0.17 | -6.65 ± 0.51 | -6.75 ± 0.05 | -6.62 ± 0.02 | -7.50 ± 0.04 | -7.52 ± 0.06 | -8.02 ± 0.19 | > -5.00 |
| Spironolactone | -8.80 ± 0.41 | -8.36 ± 0.26 | -6.37 ± 0.10 | -6.17 ± 0.19 | -6.72 ± 0.12 | -6.65 ± 0.04 | -7.52 ± 0.12 | > -5.00 |
| Dexamethasone | -7.58 ± 0.31 | -7.59 ± 0.27 | n.c. | n.c. | n.c. | n.c. | > -5.00 | > -5.00 |
| Beclomethasone | -7.59 ± 0.41 | -7.70 ± 0.10 | n.c. | n.c. | n.c. | n.c. | > -5.00 | n.d. |
| Betamethasone | -6.62 ± 0.04 | -7.15 ± 0.26 | n.c. | n.c. | n.c. | n.c. | > -5.00 | n.d. |
| Cortisol | -6.35 ± 0.11 | -6.49 ± 0.04 | n.c. | n.c. | n.c. | n.c. | > -5.00 | -8.22 |
| Triamcinolone | -6.37 ± 0.13 | -6.56 ± 0.19 | n.c. | n.c. | n.c. | n.c. | > -5.00 | n.d. |
| Prednisolone | -6.61 ± 0.09 | -7.10 ± 0.38 | n.c. | n.c. | n.c. | n.c. | n.d. | n.d. |
| Methyl-prednisolone | -7.60 ± 0.43 | -7.48 ± 0.30 | n.c. | n.c. | n.c. | n.c. | n.d. | -8.75 |
| Mifepristone | -5.81 ± 0.05 | -5.86 ± 0.06 | -6.15 ± 0.10 | -6.08 ± 0.03 | -6.88 ± 0.20 | -6.95 ± 0.21 | -7.89 ± 0.13 | > -5.00 |
| Corticosterone | -6.22 ± 0.08 | -6.25 ± 0.11 | n.c. | n.c. | n.c. | n.c. | > -5.00 | -8.22 |
| Aldosterone | -6.03 ± 0.04 | -6.11 ± 0.02 | n.c. | n.c. | n.c. | n.c. | > -5.00 | > -5.00 |
| 17α-progesterone | -6.45 ± 0.16 | -6.29 ± 0.03 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | n.d. |
| Progesterone | -6.13 ± 0.08 | -6.22 ± 0.12 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | -7.85 ± 0.11 | -7.55 |
| 17β-estradiole | -7.19 ± 0.20 | -7.54 ± 0.01 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | -7.30 ± 0.25 | > -5.00 |
| Genistein | -6.62 ± 0.42 | -6.79 ± 0.23 | n.c. | n.c. | n.c. | n.c. | > -5.00 | > -5.00 |
| Diethylstilbestrol | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 |
| 4-hydroxytamoxifen | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 |
| Raloxifene hydrochloride | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 |
| Tamoxifen citrate | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | > -5.00 | n.d. |
Data were calculated from triplicate measuments and are expressed as mean ± SD. Analyses were performed in three independent experiments for each clone.