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. 2015 Mar 26;10(3):e0121938. doi: 10.1371/journal.pone.0121938

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© 2015 Ishimoto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A and B) mDP were harvested until they reached confluence. The cells were then cultured with (A) or without (B) 10 mM of LiCl for seven days before being processed for the real-time RT-PCR analysis. (C-G) Effects of LiCl on the expression of osteogenic and odontogenic markers on quantitative RT-PCR. Values are the average ± SD of triplicate wells, and are representative of three independent experiments performed. (C-E) LiCl treatment significantly upregulated the expression of the odontogenic markers Dspp (*, p<0.05; C) and Klk4 (*, p<0.05; E) and the Wnt canonical marker Axin2 (*, p<0.05; D). (F) The Alp expression was not affected. (G) In contrast, LiCl treatment significantly downregulated the expression of the osteogenic markers Opn (*, p<0.05). Scale bars, 100 μm in A and B.