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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Mamm Genome. 2015 Feb 3;26(0):154–172. doi: 10.1007/s00335-015-9556-0

Figure 10.

Figure 10

Otoconial deposition and epithelial expression of otoconial proteins in A/J vs. C57 inner ears as detected by Western blotting (A) and real-time quantitative RT-PCR (B, C). In (A), total otoconia extract from one C57 and one A/J mouse at age P4 was loaded in each lane (labeled as “O”). This increase of Oc90 in A/J otoconia is confirmed by loading an equal amount of total protein in each lane (not shown). (B, C) Real-time PCR shows a significant increase in both Oc90 and Otol1 mRNA in the A/J inner ear epithelia as compared to C57 tissues at age P8 (*, P < 0.05; **, P < 0.01; ***, P < 0.001, n=3).