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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Exp Neurol. 2012 Jun 21;237(1):103–115. doi: 10.1016/j.expneurol.2012.05.020

Fig. 4.

Fig. 4

Representative images of VEGF receptor-2 (VEGFR-2) immunostaining in C4 ventral horn. VEGFR-2 is expressed in NeuN positive cells, including large, presumptive phrenic motor neurons (small black boxes). 3×wAIH significantly increased VEGFR-2 protein expression in presumptive phrenic motor neurons versus control, normoxic rats (A–B). Optical density (OD) analysis showed significant increase in VEGF-R2 immunoreactivity following 3×wAIH (C). Data are means±1 SEM. *p<0.05 versus normoxic controls. Immunofluorescence for large, NeuN positive cells confirmed that VEGFR-2 is localized in presumptive phrenic motor neurons (white circle). Further, VEGFR-2 immunoreactivity is enhanced following 3×wAIH (D–E). VEGF was not detectable in GFAP positive (green) astrocytes (F) or in OX-42 positive (green) microglia (G), even after 3×wAIH. Scale bars: 200 μm for lower magnification (A–B, D–E) and 100 μm for higher magnification (small boxes in D–E); F–G is 50 μm.