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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Hum Genet. 2015 Jan 15;60(3):119–126. doi: 10.1038/jhg.2014.114

Figure 3.

Figure 3

Effects of G169R mutation on CEACAM16 trafficking and secretion. (a, b) Confocal images of COS7 cells expressing Flag-tagged wild type (WT, a) and G169R mutant (b) CEACAM16 (green), counterstained for actin (red). Arrows in a and b show secreted proteins. (c) Western blot of protein extracts of HEK293T cells expressing wild type (WT) and G169R mutant Flag-CEACAM16 and of culture media secreted protein precipitates (upper panel). Total protein expression of WT and G169R CEACAM16 is very similar (Cells); total β-actin loads from both preparations are comparable (lower panel). However, the secreted (Medium) amount of mutant CEACAM16 is much lower than the WT CEACAM16. Scale bar: 10 μm.