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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Exp Hematol. 2014 Dec 27;43(4):319–330.e10. doi: 10.1016/j.exphem.2014.12.005

Figure 1. Generation of Hspa9+/− mice.

Figure 1

A) Exons 1–4 of the Hspa9 locus with gene trap insertion. A black bar indicates the Southern blot probe. B) Southern blot of tail DNA from Hspa9+/+ and Hspa9+/− mice showing DNA fragments from a wild-type allele (white arrow, 4817 bp) and gene trap-disrupted allele (black arrow, 3303 bp). C) Results of 3 primer PCR amplification of tail DNA from Hspa9+/+ and Hspa9+/− mice showing a band from the wild-type allele (white arrow, 453 bp) and gene trap-disrupted allele (black arrow, 307 bp). D) Expression of Hspa9 in bone marrow and spleen of littermates by C-terminal antibody with β-Actin loading control. E) RT-PCR expression of Hspa9 mRNA in bone marrow of littermates, normalized to Gapdh (N=3/genotype). Statistical analysis by two tailed Student’s t-test. Error bars represent mean ± SD.