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. 2014 Oct 14;64(4):1120–1130. doi: 10.2337/db14-1238

Figure 3.

Figure 3

Identification of KCs in mouse liver. A: Schematic diagram of the irradiation model used to distinguish KC and RHM. i.v., intravenous. B: FITC+ KCs and PKH26+ newly infiltrating macrophages from HFD mice were plotted in forward and side scatter. The scattergram is representative of five independent mice. C: Whole-liver FITC+ KC numbers from mice fed NC were compared with mice after 12 weeks of HFD feeding. Data represent mean ± SEM (n = 5–6). D: MCP-1 and CCR2 mRNA expression were measured from FACS-sorted KCs and RHMs isolated from NC and HFD mice. Data represent mean ± SEM (n = 5). *P < 0.05 compared with KC vs. RHM. E: Hepatic glucose production assay in mouse hepatocytes. Hepatocytes were cultured with conditioned media from FACS-sorted KCs and RHMs from NC (N) and HFD (H) mice. Data represent mean ± SEM (n = 5). *P < 0.05 compared with glucagon and insulin in RPMI.