Table 1.
Cell surface markers to distinguish between KCs and RHMs*
| Symbol | Description | NC | HFD | ||||
|---|---|---|---|---|---|---|---|
| KC | RHM | f(KC/RHM) | KC | RHM | f(KC/RHM) | ||
| KC marker† | |||||||
| F8 | Coagulation factor VIII | 20,355 | 4,238 | 5 | 12,074 | 1,198 | 10 |
| Kit | Kit oncogene | 11,300 | 2,186 | 5 | 5,430 | 492 | 11 |
| Wnt2 | Wingless-related MMTV integration site 2 | 4,836 | 818 | 6 | 2,580 | 233 | 11 |
| Prelp | Proline arginine-rich end leucine-rich repeat | 3,485 | 699 | 5 | 2,383 | 234 | 10 |
| Masp1 | Mannan-binding lectin serine peptidase 1 | 2,396 | 450 | 5 | 1,915 | 182 | 11 |
| Vwf | Von Willebrand factor homolog | 2,320 | 416 | 6 | 787 | 121 | 7 |
| Wnt9b | Wingless-type MMTV integration site 9B | 1,149 | 156 | 7 | 288 | 21 | 14 |
| Selp | Selectin, platelet | 973 | 178 | 5 | 1,006 | 87 | 12 |
| Cdh13 | Cadherin 13 | 929 | 114 | 8 | 982 | 64 | 15 |
| Dkk3 | Dickkopf homolog 3 (Xenopus laevis) | 911 | 156 | 6 | 447 | 49 | 9 |
| Tnxb | Tenascin XB | 828 | 154 | 5 | 577 | 41 | 14 |
| Col3a1 | Collagen, type III, α 1 | 752 | 100 | 8 | 711 | 46 | 15 |
| Fbln2 | Fibulin 2 | 355 | 61 | 6 | 397 | 32 | 12 |
| Pdgfd | Platelet-derived growth factor, D polypeptide | 343 | 66 | 5 | 231 | 23 | 10 |
| Col1a2 | Collagen, type I, α 2 | 381 | 59 | 6 | 379 | 29 | 13 |
| Col1a1 | Collagen, type I, α 1 | 357 | 50 | 7 | 423 | 20 | 21 |
| RHM marker† | |||||||
| C1qa | Complement component 1, q subcomponent, α polypeptide | 15,635 | 80,760 | 5 | 39,139 | 107,329 | 3 |
| C1qc | Complement component 1, q subcomponent, C chain | 15,459 | 74,157 | 5 | 34,129 | 98,710 | 3 |
| Apoc1 | Apolipoprotein C-I | 2,510 | 12,325 | 5 | 2,734 | 10,236 | 4 |
| C4b | Complement component 4B (Chido blood group) | 2,361 | 7,773 | 3 | 2,798 | 8,656 | 3 |
| Gbp2b | Guanylate binding protein 2b | 1,156 | 5,569 | 5 | 3,824 | 13,625 | 4 |
| C6 | Complement component 6 | 927 | 8,118 | 9 | 1,142 | 6,303 | 6 |
| Kif23 | Kinesin family member 23 | 912 | 2,633 | 3 | 1,257 | 3,436 | 3 |
| Cpq | Carboxypeptidase Q | 661 | 2,884 | 4 | 1,571 | 4,243 | 3 |
| C4a | Complement component 4A (Rodgers blood group) | 221 | 869 | 4 | 224 | 780 | 3 |
| Kcp | Kielin/chordin-like protein | 75 | 471 | 6 | 63 | 231 | 4 |
| Slc1a3 | Solute carrier family 1 (glial high affinity glutamate transporter), member 3 | 36 | 220 | 6 | 72 | 228 | 3 |
*Differentially expressed genes between KC and RHMs that were classified within the GO cellular component term “extracellular region part” are listed.
†The top 16 KC marker genes listed are expressed at least fivefold higher in KC vs. RHM from NC- or HFD-fed mice. The top 11 RHM markers are all expressed at least threefold higher in RHMs vs. KCs. All markers are expressed at 200 counts per gene or more. RNA-seq studies used three biological replicates per each group.