Skip to main content
. 2014 Oct 28;64(4):1235–1248. doi: 10.2337/db14-1164

Figure 1.

Figure 1

The expression of noncanonical Wnt signaling mediators is elevated in visceral fat of obese individuals. Visceral and subcutaneous fat samples were obtained from obese individuals at the time of bariatric surgery. A: qRT-PCR analysis of the expression of prototypical canonical (WNT3A, WNT10B) and noncanonical (WNT5A, WNT11) Wnt molecules in human fat (n = 31). *P < 0.0001, visceral vs. subcutaneous. B: Western blot analysis of the expression of WNT5A in human fat. Subcutaneous and visceral fat from 10 obese individuals were analyzed. Top: Densitometric quantification of WNT5A protein expression. Bottom: Representative immunoblot. C: WNT5A expression was analyzed by qRT-PCR and graphed as a function of patient waist-to-hip ratio (n = 31). The correlation between WNT5A mRNA expression and waist-to-hip ratio was evaluated using a Pearson test. SCF, subcutaneous fat; VF, visceral fat.