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. 2014 Oct 28;64(4):1235–1248. doi: 10.2337/db14-1164

Figure 4.

Figure 4

Myeloid-restricted Wnt5a-KO ablation is sufficient to attenuate IR and adipose tissue inflammation in obese mice. Thioglycollate-induced peritoneal macrophages were isolated from C57Bl/6J mice, and Wnt5a expression was evaluated by qRT-PCR (A) or Western blot (B). Graphs show the average of three independent experiments. SVFs of epididymal WAT were collected by enzymatic digestion from HFHS-fed Mye-Wnt5a-KO mice and WT littermates (n = 6 per genotype) and analyzed by qRT-PCR (C) and Western blot (D). E–G: Mye-Wnt5a-KO mice and WT littermates (n = 11 per genotype) were fed an HFHS diet for 12 weeks. E: GTT and corresponding AUC analysis. F: ITT and corresponding AUC analysis. G: qRT-PCR analysis of gene expression in epididymal WAT. Asterisk indicates the experimental group corresponding to the P value shown in the panel.