Figure 6.

Myeloid-restricted overexpression of Wnt5a promotes obesity-induced adipose tissue inflammation. Mye-Wnt5a-TG and WT mice (n = 4–9 mice per group) were fed a normal diet or HFHS diet for 12 weeks. A: qRT-PCR analysis of the transcript levels of IL-6, TNF-α, and CCL2/MCP-1 in epididymal WAT. B: qRT-PCR analysis of the expression of various macrophage-specific transcripts in epididymal WAT. C: Quantification of the amount of CLS in epididymal WAT of HFHS-fed mice. At least 1,000 adipocytes per mouse were analyzed. Representative images of hematoxylin/eosin-stained histological sections are shown. *P < 0.05; #P < 0.01, TG vs. WT. ND, normal diet.