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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Diabetes. 2014 Oct 16;64(4):1202–1210. doi: 10.2337/db14-0737

Figure 3. Electrophysiological analysis of TRPA1 in GLUTag cells.

Figure 3

A. Representative current-clamp recording showing the increase in action potential frequency following the application of TRPA1 agonist carvacrol (50 μM). The effect of carvacrol was reversible and did not affect responses to the subsequent application of glucose (10 mM). B. The addition of carvacrol (Car) significantly and reversibly increased action potential frequency and depolarised the plasma membrane (n=4). C. Representative current-voltage trace showing the effects of carvacrol application on increasing the outward and inward-rectifying properties of an isolated TRP-like current. The change in rectification properties was fully reversed following the co-application of A-967079 (A-9, 10 μM). D. Carvacrol caused a significant increase in the inward current at −50 mV (typical resting membrane potential) and outward current at +80 mV, which was reversed following the co-application of A-967079 (n=7, *p<0.05, **p<0.01, two-way paired Student’s t-test)