Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Diabetes. 2014 Oct 16;64(4):1202–1210. doi: 10.2337/db14-0737

Figure 4. The effect of TRPA1 agonism on GLP-1 secretion from GLUTag cells.

Figure 4

A. AITC (100 μM) incubation with GLUTag cells caused a significant increase in GLP-1 secretion over two hours, which was fully inhibited when AITC was co-incubated with HC-030031 (50 μM). B-D. The incubation of polyunsaturated fatty acids (PUFA), AA, EPA and DHA (all at 200 μM), also caused a significant and robust increase in GLP-1 secretion from GLUTag cells. The co-incubation with A-967079 significantly reduced, but did not abolish, the effect of PUFA-induced GLP-1 secretion. All experiments were n= ≥6, ***/###p<0.001, one-way ANOVA with Bonferroni post hoc test; * indicates significance level from baseline and # indicates significance level between groups. The dotted line on each graph represents the respective baseline value.