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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Chem Biol. 2009 Apr 24;16(4):461–470. doi: 10.1016/j.chembiol.2009.02.014

Figure 1. AdamantylSGC Inhibition of Yeast Hsc70 ATPase Activity-Single Turnover Assay.

Figure 1

(A) Yeast Hsc70 (Ssa1p) was preloaded with [32P] ATP ± SV40 T antigen (TAg, J-domain-containing protein) on ice and 32P release from the purified complex ± adaSGC (300 μM added at 50 s) monitored with incubation at 30°C. Means of triplicate determinations ± standard error are shown. ○ Yeast Hsc70 (Ssa1p); ● Yeast Hsc70 (Ssa1p) + 300 μM adaSGC; □ Yeast Hsc70 (Ssa1p) + TAg; ■Yeast Hsc70 (Ssap1) + TAg + 300 μM adaSGC.

(B) Inhibition of T antigen stimulated Hsc70 single-turnover ATPase activity was monitored as a function of adaSGC concentration.

(C) Structure of adamantylSGC (adaSGC).