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a Indices of cellular ROS (superoxide anion [O2•–]) change with aging and fiber type. a The fluorescent superoxide indicator dihydroethidium (HE) was used to evaluate oxidative stress in 6-, 26-, and 30-month EDL and soleus muscles. b Nitotyrosine changes with aging and fiber type. Anti-nitrotyrosine antibody was use to evaluate tyrosine nitrosylation in 6-, 26-, and 30-month EDL and soleus muscles. Bar 50 μm. n = 3 each group
