Ability of four solvents to fractionate unsaturated fatty acids (palmitoleic, oleic, linoleic, linolenic) derived from pistachio oil. Solvents tested: n-hexane (Hx), dichloromethane (DCM), ethyl acetate (EtAc) and ethanol (EtOH). Different letters indicate significant differences across unsaturated fatty acids and across solvents at P < 0.05 according to DMRT, n = 3