Column stationary phase	: Shimpak C18, S-5 μm, 120A, 250 × 4.6 mm ID
Phase	: Reverse phase
UV detector wavelength	: 200 nm
Mobile Phase (A)	: 0.02 M phosphate buffer, pH 5.0: acetonitrile	(97:3)
Mobile Phase (B)	: 0.02 M phosphate buffer, pH 3.5: acetonitrile	(80:20)
Flow rate	: 1 ml/min
Run time	: 30 min.
Maximum pressure	: 400 kgmf