Table 1.
Twelve-trial PBD to study the effects of 11 factors on the encapsulation efficiency EE (%) and activity of the liposomal Flavourzyme (LAPU−1) as responses
| Run no. | pH | Cholesterol/lecithin (w/w) | Lecithin (% w/w) | Enzyme/lecithin (w/w) | Stirring time (min) | Temperature (°C) | Speed of stirrer (rpm) | Ratio of stirrer to tank diameter | Homogenization (rpm) | Way of enzyme addition | Centrifugation condition (×1,000 g) | Encapsulation efficiencya | Activity of the liposomal flavourzyme |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 8 | 0.05 | 4 | 0.2 | 5 | 50 | 1000 | 0.9 | 10000 | A | 40 | 16.47 ± 0.96 | 0.59 |
| 2 | 8 | 0.1 | 2 | 0.1 | 5 | 50 | 500 | 0.45 | 10000 | A | 45 | 5.44 ± 0.27 | 0.61 |
| 3 | 6 | 0.1 | 4 | 0.2 | 15 | 50 | 500 | 0.9 | 0 | A | 45 | 22.21 ± 2.63 | 0.67 |
| 4 | 8 | 0.05 | 4 | 0.1 | 5 | 60 | 500 | 0.9 | 0 | B | 45 | 8.73 ± 1.10 | 0.14 |
| 5 | 8 | 0.1 | 2 | 0.1 | 15 | 50 | 1000 | 0.9 | 0 | B | 40 | 9.33 ± 0.54 | 0.10 |
| 6 | 8 | 0.1 | 4 | 0.2 | 15 | 60 | 500 | 0.45 | 10000 | B | 40 | 5.83 ± 0.89 | 0.16 |
| 7 | 6 | 0.1 | 4 | 0.1 | 5 | 60 | 1000 | 0.45 | 0 | A | 40 | 8.82 ± 1.43 | 0.17 |
| 8 | 6 | 0.05 | 4 | 0.1 | 15 | 50 | 1000 | 0.45 | 10000 | B | 45 | 16.44 ± 0.97 | 0.29 |
| 9 | 6 | 0.05 | 2 | 0.1 | 15 | 60 | 500 | 0.9 | 10000 | A | 40 | 9.27 ± 2.01 | 0.1 |
| 10 | 8 | 0.05 | 2 | 0.2 | 15 | 60 | 1000 | 0.45 | 0 | A | 45 | 9.33 ± 2.1 | 0.16 |
| 11 | 6 | 0.1 | 2 | 0.2 | 5 | 60 | 1000 | 0.9 | 10000 | B | 45 | 4.99 ± 0.32 | 0.1 |
| 12 | 6 | 0.05 | 2 | 0.2 | 5 | 50 | 500 | 0.45 | 0 | B | 40 | 7.55 ± 0.54 | 0.13 |
aConfidence limit: 99 %