Table 3.
Variables | EE (%) | Activity of the liposomal flavourzyme (LAPU−1) | ||||||
---|---|---|---|---|---|---|---|---|
Effect | Coefficient | t-value | p-value Prof>F | Effect | Coefficient | t-value | p-value Prof>F | |
X1: pH | −2.08 | −1.04 | −4.17 | 0.00 | −0.04 | −0.02 | −2.42 | 0.00 |
X2: cholesterol/lecithin (% w/w) | −1.84 | 0.92 | 3.71 | 0.001 | −0.02 | −0.01 | −1.51 | 0.14 |
X3: lecithin (% w/w) | 5.00 | 2.50 | 10.04 | 0.00 | 0.23 | 0.11 | 13.6 | 0.00 |
X4: enzyme/lecithin ratio (% w/w) | −1.48 | −0.74 | −2.98 | 0.005 | −0.14 | −0.06 | −8.27 | 0.00 |
X5: stirring time (min) | 3.34 | 1.67 | 6.70 | 0.00 | 0.05 | 0.02 | 2.94 | 0.00 |
X6: temperature (°C) | −4.19 | −2.09 | −8.42 | 0.00 | −0.15 | −0.07 | −9.08 | 0.00 |
X7: speed of stirrer (rpm) | 0.98 | 0.49 | 2.00 | 0.05 | 0.01 | 0.00 | 0.48 | 0.63 |
X8: homogenization | −0.97 | −0.48 | −1.96 | 0.06 | −0.03 | −0.01 | −1.75 | 0.08 |
X9: way of enzyme addition | 3.23 | 1.61 | 6.48 | 0.00 | 0.12 | 0.06 | 7.04 | 0.00 |
X10: centrifugation condition | 1.18 | 0.59 | 2.39 | 0.02 | 0.04 | 0.02 | 2.71 | 0.01 |
X11: ratio of stirrer to tank diameter | 2.52 | 1.26 | 5.07 | 0.00 | 0.11 | 0.06 | 7.06 | 0.00 |
Each value is the average of four replications
Confidence limit: 99 %