Fig. 4.

CM-Toyoperal 650 M column chromatography of denatured emu skin PSC. A 1.0 × 6.0 column of CM-Toyopearl 650 M was equilibrated with 20 mM sodium acetate buffer (pH 4.8) containing 6 M urea, and maintained at 37 °C. The PSC (10.0 mg) was dissolved in 5 ml of the same buffer, denatured at 45 °C for 30 min, and then eluted from the column with a linear gradient of 0 to 0.15 M NaCl at a flow rate of 0.8 ml/min. The fractions indicated by the numbers were examined by SDS-PAGE using 7.5 % gel