Table 1.

Effect of tie line length (TLL, %) on the change in free volume and partitioning

PEG molecular weight (Da)	TLL (%)	Phase volume ratio, Vr (−)	Change in free volume, ∆Vf (10−3 ml/g)	KCPC	KTP	Purity of CPC (−)	Yield of CPC (%)
1500	17	1.05	41.6	28.9	1.1	2.84 ± 0.2a	~100 a
	21	1.07	60.7	51.3	1.3	2.71 ± 0.1b	96.8 ± 0.2b
	24	1.20	89.0	44.00	2.4	1.47 ± 0.09c	90.1 ± 0.7c
4000	10	0.64	37.36	13.9	1.1	3.56 ± 0.07a	92.5 ± 0.2a
	14	0.59	38.03	16.7	1.4	3.37 ± 0.02 a	94.4 ± 08b
	19	0.53	42.92	19.1	1.7	3.26 ± 0.08 a	90.0 ± 0.7c
	23	0.27	106.06	37.7	2.5	3.30 ± 0.02 a	67.5 ± 0.2d
	30	0.14	109.32	39.7	6.4	2.13 ± 0.07 b	67.0 ± 0.9d
6000	16	0.77	76.7	2.5	2.5	2.52 ± 0.02a	52.5 ± 0.6a
	20	0.50	78.9	5.2	3.6	2.14 ± 0.05a	67.6 ± 0.9b
	24	0.31	83.4	27.0	9.4	2.51 ± 0.08a	65.3 ± 0.8c

Means of the same column followed by different letters differ significantly (p < 0.05), Purity of CPC in crude extract was 1.0