FIG 5.

Purified amylosin causes dose-dependent potassium ion efflux from human primary PBMC, cultured keratinocytes (HaCaT), and porcine spermatozoa. Washed PBMC (20 × 10⁶ cells ml⁻¹) (A and B), HaCaT cells (6 × 10⁶ cells ml⁻¹) (C and D), or spermatozoa (54 × 10⁶ cells ml⁻¹) (E) were placed in isotonic potassium-free medium in a measurement chamber. The traces show the extracellular [K⁺] before and during exposure to the indicated concentrations of amylosin (A, C, and E) or alamethicin (B and D), recorded with a K⁺-selective electrode (one reading per second). The time points of amylosin and alamethicin additions are indicated by arrows.