FIG 7.
Amylosin-producing B. amyloliquefaciens 19b, isolated from a microbially damaged indoor space, inhibited the growth of fungi and bacteria isolated from similarly damaged indoor spaces and plated on TSA plates. (A and B) Two TSA plates were inoculated with a spore suspension of Chaetomium globosum MTAV 35 (an indoor fungus) and grown for 1 day, after which a horizontal streak of B. amyloliquefaciens 19b was drawn across one of the plates (B). Upon further culturing (10 days), the spores of C. globosum MTAV 35 failed to germinate in the vicinity of the streaked B. amyloliquefaciens 19b. The inhibition zone was 12 mm wide. (C to E) Six bacterial strains were horizontally streaked on three TSA plates: 1, Williamsia muralis MA140/96T; 2, Mycobacterium murale MA113T; 3, Dietzia sp. MA147; 4, Sphingomonas aurantiaca MA101bT; 5, Bacillus sp. OS16; 6, Bacillus megaterium Ne10. Vertical streaks of B. amyloliquefaciens 19b (strain 8, amylosin producer) or Bacillus cereus NS58 (strain 7, cereulide producer) were drawn vertically across plates D and E, respectively.