FIG 6.

Growth phenotype of methionine-auxotrophic C. glutamicum strains on MetO with or without H₂O₂ challenge. Overnight-grown ΔmetH ΔmetE(pXMJ19), ΔmetH ΔmetE ΔmshC(pXMJ19), ΔmetH ΔmetE ΔmshC(pXMJ19-mshC), ΔmetH ΔmetE ΔmsrA(pXMJ19), and ΔmetH ΔmetE ΔmsrA(pXMJ19-msrA) strains (optical density at 600 nm [OD₆₀₀] = 1.6 to 1.7) were spun down, washed twice with mineral salts medium, and resuspended in mineral salts medium, and then, 5 μl of serial dilutions (from left to right) were spotted on solid mineral salts medium plates containing 0.5 mM IPTG, 10 μg/ml MetO (+MetO), or 10 μg/ml MetO and 5 mM H₂O₂ (+MetO+H₂O₂). A control assay shows all cells growing equally on minimal medium plates containing 10 μg/ml methionine (+Met). The plates were incubated at 30°C for 2 days and then photographed.