TABLE 3.
Sodalis HemR enables use of hemin and hemoglobin as iron sources in E. coli strain UR45
| Plasmid (gene) carried by UR45 | Mean diam (mm) with indicated iron sourcea |
||||
|---|---|---|---|---|---|
| FeSO4 (10 mM) | H2O | Hemin |
Hb (1 μM) | ||
| 5 μM | 50 μM | ||||
| pWKS30 | 10 ± 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| pTA1 (hemR) | 11 ± 1 | 0 ± 0 | 9 ± 1 | 12 ± 1 | 8 ± 1 |
| pTA2 (hemR and hemTUV) | 10 ± 0 | 0 ± 0 | 8 ± 2 | 15 ± 1 | 9 ± 1 |
a
E. coli UR45 (entF::cam dppB::kan) containing the indicated plasmid was seeded (106 bacteria/ml) onto L agar plates containing 100 μg/ml EDDA and 125 μg/ml carbenicillin. Five microliters of each indicated iron source was spotted directly onto the agar. The size of the diameter of growth stimulation around the spotted compound was measured after 24 h of incubation at 37°C. The data shown are the means ± standard deviations of three experiments. Hb, hemoglobin.