TABLE 3.
The numbers of compounds supplemented as the sole source of carbon, nitrogen, phosphate, or sulfur differentially metabolized between KT2440(pCAR1) and NAP gene disruptantsa
| Strain | No. of C sources (PM1 and PM2; 190 compounds) | No. of N sources (PM3; 95 compounds) | No. of P sources (PM4; 59 compounds) | No. of S sources (PM4; 35 compounds) | Total no. of sources |
|---|---|---|---|---|---|
| KT2440(pCAR1)b | 22 (3, 19) | 18 (1, 17) | 22 (5, 17) | 4 (0, 4) | 66 (9, 57) |
| KT2440 (pCAR1Δpmr)c | 13 (13, 0) | 4 (4, 0) | 5 (2, 3) | 1 (1, 0) | 23 (20, 3) |
| KT2440 (pCAR1Δpnd)c | 10 (8, 2) | 4 (4, 0) | 8 (5, 3) | 0 | 22 (17, 5) |
| KT2440 (pCAR1Δphu)c | 10 (9, 1) | 1 (0, 1) | 4 (0, 4) | 0 | 15 (9, 6) |
| KT2440 (pCAR1ΔpmrΔpnd)c | 11 (9, 2) | 1 (0, 1) | 6 (0, 6) | 0 | 18 (9, 9) |
| KT2440 (pCAR1ΔpmrΔphu)c | 10 (7, 3) | 2 (0, 2) | 7 (5, 2) | 1 (1, 0) | 20 (13, 7) |
a
Two independent analyses were performed with each strain for each compound, and cellular respiration activity was compared among the four combinations. The numbers in parentheses in columns 2 to 6 indicate the numbers of compounds with which cell respiration increased (left) or decreased (right).
b
The results from KT2440(pCAR1) were compared with those from KT2440 (9).
c
The results from NAP gene disruptants were compared with those from KT2440(pCAR1).