TABLE 3.
Fold difference in mRNA transcripts for c-type cytochrome proteins
| Locus ID | No. of heme-binding motifs | Predicted size of protein (kDa) | Resulta by: |
|
|---|---|---|---|---|
| Microarray | qRT-PCR | |||
| Upregulated genes | ||||
| Ferp_0670 | 35 | 189.28 | 3.67 | 13.24 ± 3.31 |
| Ferp_0668 | 4 | 25.59 | 2.69 | 7.88 ± 2.55 |
| Ferp_1336 | 2 | 31.91 | 2.55 | 5.26 ± 1.38 |
| Ferp_1267 | 4 | 17.73 | 2.41 | 17.80 ± 5.36 |
| Ferp_0672 | 31 | 180.93 | 2.28 | 6.94 ± 2.42 |
| Ferp_1814 | 5 | 20.80 | 2.13 | 3.56 ± 1.22 |
| Ferp_1255 | 2 | 15.67 | 2.08 | 8.65 ± 2.34 |
| Ferp_1813 | 5 | 21.20 | 2.08 | 4.56 ± 2.10 |
| Downregulated genes | ||||
| Ferp_0660 | 6 | 22.89 | −2.20 | −4.21 ± 1.98 |
| Ferp_2064 | 5 | 31.01 | −2.34 | −5.94 ± 2.67 |
| Ferp_1341 | 1 | 25.21 | −2.77 | −6.33 ± 2.35 |
a
Shown are fold differences in mRNA transcripts for c-type cytochrome proteins that were significantly upregulated or downregulated in F. placidus cells grown with acetate as an electron donor and Fe(III) oxide compared to Fe(III) citrate as an electron acceptor.