FIG 1.
Primers sets developed for sequence-specific LAMP and real-time PCR. The LAMP primer set ID:18 and ID:25 sequences are shown going from 5′ to 3′ with the F3, B3, FIP, and BIP nomenclature derived from Notomi et al. (14). The annealing positions corresponding to the A. thaliana chalcone synthase (CHS) complete coding sequence (GenBank accession number M20308.1), GC content (GCrate), 5′ and 3′ gene positions, free energy (5′dG/3′dG), and melting temperatures (Tm) are indicated. Also shown are the forward and reverse primer sequences (5′ to 3′), along with the fluorescent hybridization probe sequence, used for real-time PCR.