Sensitivity and specificity of LAMP primers for pET32(a)CHS template. We serially diluted plasmid encoding A. thaliana chalcone synthase (18) [pET32(a)CHS] or genomic DNA from Trypanosoma brucei subspecies, Babesia microti, Plasmodium falciparum, Toxoplasma gondii, E. coli, Rickettsia parkeri, bacteremic human patients, and normal human blood and assessed LAMP by monitoring turbidity in real-time. We used primer set ID:18 to direct LAMP in these reactions.