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Time course of geometric mean anti-PA IgG levels (μg/ml) by group as determined by ELISA. All animals received the initial vaccination on study day 0. Groups receiving a second vaccination (groups 5, 6, and 7) had vaccine administered on study day 28. Values less than the limit of quantification (5 μg/ml) were assigned a value of 2.5 μg/ml. Error bars, 95% confidence intervals of the geometric means; arrows, times of vaccination (v) and challenge (c); GMT, geometric mean titer.
