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Frequency distribution of the negative sera tested using the 3B cELISA for serum samples collected from disease-free cattle (n = 503), pigs (n = 508), and sheep (n = 507) in Canada (a) and cattle (n = 353) and pigs (n = 436) in Mexico (b). Recombinant 3ABC protein was coated onto microtiter plates. Equal volumes of diluted sera and MAb (F8-3Bp) were added to the plates and allowed to compete with antibodies in the serum samples. Results are expressed as percent inhibition.
