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. 2014 Jul 25;104(1):116–126. doi: 10.1093/cvr/cvu173

Figure 5.

Figure 5

NO-cGMP signalling results in the induction of β-catenin-dependent target genes. HUVECs were treated with LiCl (10 mmol/L), spermine NONOate (NO; 10 µmol/L), or sildenafil (Sil; 100 nmol/L) for 12 h. Transcript levels of IL-8 (A) and cyclin D1 (B) were assessed by quantitative RT–PCR using HPRT as a housekeeping gene and quantified using the ΔΔCt method and shown relative to vehicle (*P < 0.05 vs. vehicle, one-way ANOVA with repeated measures; n = 4 per group for cyclin D1 and n = 5 per group for IL-8).