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. 2014 Dec 23;106(1):143–152. doi: 10.1093/cvr/cvu262

Figure 5.

Figure 5

Neuronal Na+ channel blockade desynchronizes DCR in tissue. Representative Ca2+ (top) and voltage (bottom) line-scan images of Iso-treated R33Q muscle preparations paced at 1 Hz before (A) and after (B) exposure to riluzole (Ril, 10 µM), along with the corresponding Rhod-2 fluorescence profiles from each myocyte and average di-4-ANBDQBS signal. Dashed white lines outline myocyte borders. Red asterisks indicate tissue-wide extrasystolic Ca2+ release (DCR) that resulted in triggered activity (TA). (C) Temporal distribution of DCR in cardiac muscles (n = 264 events for Iso alone, n = 355 for Ril and n = 372 for 100 nM TTX). (D) Median latencies to first DCR (solid bars) and their S.D. (hashed bars) were increased by Ril (n = 355 events) and TTX (n = 372 events) relative to Iso alone (n = 264 events). (E) DCRs and TA frequency per second (Hz) before and after treatment with Ril (10 µM; n = 26 cells from four preparations isolated from three mice, *P < 0.05) or TTX (100 nM; n = 29 cells from three preparations isolated from three mice, *P < 0.05).