Figure 2.

Rate constant for tension redevelopment. k_tr is plotted as a function of normalized tension for fibers treated with low LKB1 activation of AMPK (rAMPK/LKB1^lo), high LKB1 activation of AMPK (rAMPK/LKB1^hi), and LKB1 complex alone (LKB1) plotted relative to untreated fibers. (A–C) Data were binned and fitted to a curvilinear fit. There is a significant increase in the slope of rAMPK/LKB1^lo-treated fibers compared with untreated fibers (p < 0.05). rAMPK/LKB1^hi-treated fibers tend to have an increased slope compared with untreated fibers. There is no difference between curvilinear slopes between LKB1-complex-treated fibers and untreated fibers. (D–F) Raw data were fitted with a linear regression, and multiple linear regression analysis was used to measure differences between slopes. There is a significant increase in the slope in fibers treated with rAMPK/LKB1^lo (p < 0.05). There is a tendency for an increased slope with rAMPK/LKB1^hi-treated fibers, with a significantly different intercept (p < 0.05) compared with untreated fibers. There is no difference in linear slopes between LKB1-complex-treated and untreated fibers.