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. 2015 Mar 10;108(5):1199–1212. doi: 10.1016/j.bpj.2015.01.014

Figure 5.

Figure 5

Effect of lysine acetylation on the rate of fibrillization of WT- and ALS-variant apo-SOD1 in 100 mM NaCl. Thioflavin-T fluorescence assays of unacetylated and acetylated (A) WT, (B) D90A, and (C) A4V apo-SOD1 proteins (pH 7.4, 37°C). Each curve is an average of data (normalized) from 18 separate replicate experiments. The mean number of acetylated lysines is denoted as Lys-Ac(∼N), per apo-SOD1 monomer. (D–F, upper panels) Transmission electron micrographs of fibrillar forms of acetylated WT, D90A, and A4V apo-SOD1 after the ThT assay in 100 mM NaCl. (D–F; lower panels) SDS-PAGE of samples before (left) and after (right) ThT aggregation assay in 100 mM NaCl. The gels on the right in each panel are from supernatants of samples after aggregation and centrifugation. Table 1 and Fig. S13 list p-values for all kinetic analyses extracted from ThT fluorescence assays. To see this figure in color, go online.